BIO-181L Pre-lab Worksheet: Enzyme In this lab, we will study several factors that affect the activity of enzymes. Enzymes are made up of amino acids and have optimal working conditions. Enzymes act as catalysts for reactions, they help lower the activation energy of chemical reactions, helping the conservation of energy when those chemical reactions happen. Enzymes are very specific to different proteins or substrates, also enzymes are affected by temperature, pH, and concentrations. We will be working with the catechol oxidase enzyme, isolated from a potato.

Hypothesis Specificity of Substrate Null hypothesis: Enzyme activity will decrease in the presence of 6-catechol and increase with the presence of 1-distilled water. Alternate hypothesis: Enzyme activity will increased in...